Tthssb
WebJul 27, 2006 · TthSSB-255 could only enhance RCA reactions drastically, which showed weak ssDNA binding affinity. This characteristic of the protein would be critical for reduction of nonspecific products. As shown in Supplementary Figure SP2, TthSSB has a significant cluster consisting of acidic residues in its C-terminus although the role has not been … WebThe presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus. TthSSB was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate Tth DNA polymerase during cDNA synthesis. National Category Biochemistry and Molecular Biology Identifiers
Tthssb
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WebFurthermore, TthSSB increases the fidelity of proofreading deficient Thermus thermophilus DNA polymerase (Perales et al., 2003). We tested the slippage properties of PabPolB, ... WebssDNA Binding Proteins. Product Listing Product Overview. NEB offers highly thermostable ssDNA binding proteins that are ideal for nucleic acid amplification and sequencing (Tth …
WebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical … WebOct 15, 2010 · The results showed that TmaSSB and TneSSB are the most thermostable SSB proteins identified to date, offering an attractive alternative to TaqSSB and TthSSB in …
WebA fixed quantity (20 pmol) of 5h-end fluoresceinlabelled oligonucleotide (dT) 30 was incubated with 20 pmol of TaqSSB or TthSSB (#) in 20 µl reaction mixtures in standard … WebNov 15, 2003 · The thermostability of TthSSB was also studied. To this end, TthSSB was pre‐incubated at 94°C for different times, cooled down to 70°C, and immediately subjected to bandshift assays. As shown in Figure 2 C, TthSSB could withstand up to 2 min incubation at 94°C, without any detectable loss of its ssDNA‐specific binding activity.
WebNov 1, 2003 · Europe PMC is an archive of life sciences journal literature.
WebHere we show, using single-molecule fluorescence resonance energy transfer (FRET), that homodimeric bacterial SSB from Thermus thermophilus (Tth) is able to diffuse spontaneously along ssDNA over a wide range of salt concentrations (20-500mM NaCl), and that TthSSB diffusion can help transiently melt the DNA hairpin structures. income based apartments san antonio txhttp://www.biotools.eu/documentospdf/DNA-Polymerase-030.pdf income based apartments san diego cahttp://www.diva-portal.org/smash/record.jsf?pid=diva2:658627 income based apartments san marcos caWebdate, offering an attractive alternative to TaqSSB and TthSSB in molecular biology applications, especially with using high temperature e. g. polymerase chain reaction (PCR). Background Single-stranded DNA-binding (SSB) proteins play an essential role in allin vivo processes involving ssDNA. They interact with ssDNA and RNA, in an independent income based apartments san ramon caWebTthSSB appears to be positioned near the center of the (dT)60+n ssDNA overhangs though rapid diffusion. (A) A schematic illustration of our experimental design for (dT)60+n (n = 0, … income based apartments sevierville tnWebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not … income based apartments sanfordWebMay 12, 2009 · Use of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500x. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over … income based apartments seminole county