WebThermo Scientific™ Accucore™ HILIC columns are recommended for the analysis of basic or neutral polar compounds. Catecholamines Column: Accucore HILIC, 2.6µm, 50 x 2.1mm Mobile Phase: 85:15 (v/v) acetonitrile / 100mm ammonium formate (pH 3.2) Flow Rate: 2mL/min Temperature: 40°C Detection: UV at 280nm Injection Volume: 5µL Backpressure: … WebApr 29, 2011 · Stationary and mobile phases in hydrophilic interaction chromatography: a review Stationary and mobile phases in hydrophilic interaction chromatography: a review …
Stationary and mobile phases in hydrophilic interaction
WebHydrophilic interaction chromatography (HILIC) is one of the most useful analytical techniques for separating polar analytes.1,2Employing a polar stationary phase and a less polar mobile phase, retention in HILIC increases with increasing analyte polarity. WebAug 29, 2010 · To investigate the separation abilities of the sugar-based silica gel in the HILIC mode, chromatographic studies were performed with polar analytes as probes. A comparison was made with an aminopropyl-bonded silica (APS) column, which is the first bonded stationary phase to be routinely used for carbohydrate separations in the HILIC … fishing planet saint croix white bass
HILIC Method Development in a Few Simple Steps
WebFeb 12, 2024 · For a long time, HILIC has been a slight mystery. Most experts believed that the mechanism which made HILIC work was the thin water layer that developed on the … WebThis stationary phase readily and selectively retains polar compounds such as glycans. In addition, this sorbent possesses a weakly basic surface allowing the added potential to exploit ion exchange/repulsion properties. The eluent pH and ionic strength can impact retention on this HILIC phase and cannot be ignored. WebHILIC can be described as a variation of reversed phase chromatography performed using a polar stationary phase. The mobile phase employed is highly organic in nature (> 60-70% … fishing planet sainte croix